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AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsKarl Deisseroth, Ofer Yizhar, Lisa Gunaydin, Peter Hegemann, Andre Berndt
Estimating the term…
Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated molecule comprising a nucleic acid sequence that codes for light-activated molecule. The light-activated molecule includes a modification to a location near the all-trans retinal Schiff base, e.g., to extends the duration time of the open state. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion channels or pumps or for controlling currents in a cell (e.g., in vivo or in vitro environments).
-5. (canceled)
A nucleic acid comprising a nucleotide sequence encoding a light-responsive ion channel comprising an amino acid sequence having at least 85% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:1, wherein the ion channel exhibits an extended conducting state after exposure to a light pulse, and wherein the ion channel comprises a substitution of a Cys residue at a position corresponding to amino acid residue 128 (C128) of SEQ ID NO:1 and a substitution of an Asp residue at a position corresponding to amino acid residue 156 (D156) of SEQ ID NO:1.
-30. (canceled)
A system for controlling action potential of a neuron, the system comprising a delivery device, a light source, and a control device, wherein said delivery device comprises a light-responsive ion channel comprising an amino acid sequence having at least 85% amino acid sequence identity to the amino acid sequence set forth in SEQ ID No. 1, wherein the ion channel exhibits an extended conducting state after exposure to a light pulse, and wherein the ion channel comprises a substitution of a Cys residue at a position corresponding to amino acid residue 128 (C128) of SEQ ID NO:1 and a substitution of an Asp residue at a position corresponding to amino acid residue 156 (D156) of SEQ ID NO:1, or a nucleic acid encoding the light-responsive ion channel, for delivery of a light-responsive ion channel protein to a neuron by one of transfection, transduction and microinjection.
A method of stimulating a target cell in vivo, the method comprising exposing a target cell that expresses a light-responsive ion channel to an optical stimulus, thereby stimulate the target cell, wherein the light-responsive ion channel comprises an amino acid sequence having at least 85% amino acid sequence identity to the amino acid sequence set forth in SEQ ID No. 1, wherein the ion channel exhibits an extended conducting state after exposure to a light pulse, and wherein the ion channel comprises a substitution of a Cys residue at a position corresponding to amino acid residue 128 (C128) of SEQ ID NO:1 and a substitution of an Asp residue at a position corresponding to amino acid residue 156 (D156) of SEQ ID NO:1.
Dependencies are inferred from the claim text.