microRNA Biomarkers for Human Breast and Lung Cancer

A pharmaceutical composition for treating lung cancer comprising

• a) an oligonucleotide that
• i) down-regulates the over-expression of the miRNA or pre-miRNA of SEQ ID NO:4, 26, 27, or 28 in lung cancer, or
• ii) increases the levels of the miRNA or pre-miRNA of SEQ ID NO:1, 3, 19, 20, 21, 22, 23, 24, or 29 in lung cancer;
• and
• b) a second active ingredient for prophylactic or therapeutic treatment of lung cancer.

A pharmaceutical composition for treating breast cancer comprising

• a) an oligonucleotide that
• i) down-regulates the over-expression of the miRNA or pre-miRNA of SEQ ID NO:4, 5, 6, 7, 8, 12, 13, 14, 15, 16, 17, or 18 in breast cancer, or
• ii) increases the levels of the miRNA or pre-miRNA of SEQ ID NO:1, 2, 3, 9, 10, or 11 in breast cancer;
• and
• b) a second active ingredient for prophylactic or therapeutic treatment of breast cancer.

A method for detecting, classifying, diagnosing or prognosing lung cancer comprising:

• a) obtaining at least one lung sample from an individual,
• b) detecting the presence or absence of expression and/or the expression level of at least one nucleic acid molecule in cells of the sample, said nucleic acid molecule comprising:
• i) a nucleotide sequence selected from the group consisting of SEQ ID NOs:
• 1, 3, 4, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, and 29 and/or,
• ii) a nucleotide sequence which is complementary to i) and/or,
• iii) a nucleotide sequence which is a fragment of or ii) and/or,
• iv) a nucleotide sequence which has an identity of at least 80% to a sequence of i), ii) or iii) and/or,
• v) a nucleotide sequence which hybridizes under stringent conditions to a sequence of i), ii), iii) or iv), for the detection of lung cancer in said individual.

A method for detecting, classifying, diagnosing or prognosing breast cancer comprising:

• a) obtaining at least one breast sample from an individual,
• b) detecting the presence or absence of expression and/or the expression level of at least one nucleic acid molecule in cells of the sample, said nucleic acid molecule comprising:
• i) a nucleotide sequence selected from the group consisting of SEQ ID NO:5, 6, 7, 8, 9, 13, 14, 15, 16, and 18 and/or,
• ii) a nucleotide sequence which is complementary to i) and/or,
• iii) a nucleotide sequence which is a fragment of i) or ii) and/or,
• iv) a nucleotide sequence which has an identity of at least 80% to a sequence of i), ii) or iii) and/or,
• v) a nucleotide sequence which hybridizes under stringent conditions to a sequence of i), ii), iii) or iv), for the detection of lung cancer in said individual.