Improved capping modules for designed ankyrin repeat proteins
AssigneeMolecular Partners AG
InventorHans Kaspar Binz
Life of this patent
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Abstract
Improved N-terminal capping modules for designed ankyrin repeat proteins (DARPins) conferring improved thermal stability to the DARPins are described, as well as nucleic acids encoding such proteins, pharmaceutical compositions comprising such proteins and the use of such proteins in the treatment of diseases.
Claims21
1-16. (canceled)
A binding protein comprising at least one ankyrin repeat domain, wherein said ankyrin repeat domain comprises an N-terminal capping module having an amino acid sequence
- GSDLGKKLLE AARAGQDDEV RILLKAGADV NA (SEQ ID NO:14) or
- GSDLGKKLLE AARAGQDDEV RELLKAGADV NA (SEQ ID NO:15), wherein
- the amino acid residue L at position 24 of SEQ ID NO:14 or SEQ ID NO:15 is optionally replaced by V, I or A;
- up to 9 amino acids of SEQ ID NO:14 or SEQ ID NO:15 in other positions than position 24 are optionally exchanged by any amino acids;
- and
- wherein G at position 1 and/or S at position 2 of SEQ ID NO:14 or SEQ ID NO:15 are optionally missing.
A binding protein comprising at least one ankyrin repeat domain, wherein said ankyrin repeat domain comprises an N-terminal capping module having an amino acid sequence with at least 70% amino acid sequence identity with
- GSDLGKKLLE AARAGQDDEV RILLKAGADV NA (SEQ ID NO:14) or
- GSDLGKKLLE AARAGQDDEV RELLKAGADV NA (SEQ ID NO:15), and with the condition that the amino acid residue in position 24 in the amino acid sequence of said N-terminal capping module is L, V, I or A;
- and such N-terminal capping molecules wherein the amino acids in position 1 and/or 2 are missing.
A binding protein comprising at least one ankyrin repeat domain, wherein said ankyrin repeat domain comprises a C-terminal capping module having an amino acid sequence X1DKX2GKTX3X4D X5X6X7DX8GX9EDX10 AEX11LQKAA (SEQ ID NO:6), wherein
- X1 represents an amino acid residue Q or K;
- X2 represents an amino acid residue A, S or F;
- X3 represents an amino acid residue A or P;
- X4 represents an amino acid residue A or F;
- X5 represents an amino acid residue I or L;
- X6 represents an amino acid residue S or A;
- X7 represents an amino acid residue I or A;
- X8 represents an amino acid residue A, E or N;
- X9 represents an amino acid residue N or H;
- X10 represents an amino acid residue L or I;
- X11 represents an amino acid residue I or V;
- and
- wherein X2 does not represent F if X4 represents F and X7 represents I and X8 represents N or E.
Dependencies are inferred from the claim text.