Device for Nucleic Acid Analysis and Nucleic Acid Analyzer

A method for producing a reaction device for nucleic acid analysis, comprising the steps of:

• (i) forming a sample-immobilizing layer on a substrate;
• (ii) immobilizing a nucleic acid sample or a carrier having a nucleic acid sample on its surface on the sample-immobilizing layer;
• and
• (iii) introducing a blocking layer that covers an area other than an area where the nucleic acid sample or the carrier is bound to the sample-immobilizing layer, wherein
• the sample-immobilizing layer is formed of an inorganic oxide.

A nucleic acid analyzer for nucleic acid analysis, comprising:

• a reaction device for nucleic acid analysis that comprises at least a substrate,
• a sample-immobilizing layer on the substrate, a nucleic acid sample or a carrier having a nucleic acid sample on its surface, which is immobilized on the sample-immobilizing layer, and a blocking layer that covers an area other than an area where the nucleic acid sample or the carrier is bound to the sample-immobilizing layer, wherein the sample-immobilizing layer is formed of an inorganic oxide;
• a reagent supply unit for supplying a solution that contains a reagent to the reaction device for nucleic acid analysis;
• and
• a detection unit for observation of a sample retained in the reaction device for nucleic acid analysis, wherein
• the sample-immobilizing layer is formed of an inorganic oxide.