Protein Arrays and Methods of Using and Making the Same
AssigneeGen9, Inc.
InventorsJoseph Jacobson, Michael E. Hudson, Larry Li-Yang Chu
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Abstract
Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.
Claims19
A method for preparing a protein array having a plurality of proteins, the method comprising:
- (a) providing a plurality of nucleic acids each having a predefined sequence;
- and
- (b) expressing in vitro a plurality of proteins from the plurality of nucleic acids, wherein the plurality of proteins are expressed on an array.
A method for preparing a protein array having a plurality of proteins, the method comprising:
- (a) providing a first microvolume comprising a population of nucleic acids having a plurality of distinct, predefined sequences;
- (b) immobilizing the nucleic acid sequences onto an array comprising a plurality of anchor oligonucleotides having a sequence complementary to a terminus sequence of the nucleic acids;
- (c) expressing in vitro in a second microvolume a plurality of proteins from the population of nucleic acids.
A method for producing at least one protein, the method comprising:
- (a) providing a support having a plurality of distinct features, each feature comprising a plurality of immobilized anchor oligonucleotides;
- (b) generating at least one plurality of nucleic acid having a predefined sequence onto the plurality of anchor oligonucleotides;
- (c) providing a microvolume onto at least one feature of the support;
- and
- (d) expressing in vitro in the microvolume the at least one protein from the at least one nucleic acid.
A protein array comprising:
- (a) a solid surface having a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids;
- and
- (b) a microvolume covering each of the plurality of anchor oligonucleotides, the microvolume configured to produce a polypeptide from each of the plurality of nucleic acids.
A protein array comprising:
- (a) a first plurality of features on a support, each of the first plurality of features comprising a plurality of immobilized single stranded oligonucleotides, wherein the plurality of single stranded oligonucleotides comprises partially overlapping sequences that define the sequence of each of a plurality of nucleic acid molecules encoding a plurality of proteins;
- and
- (b) a second plurality of features, the second plurality of features comprising a plurality of anchor oligonucleotides having a sequence complementary to a terminus sequence of each of the plurality nucleic acids.
Dependencies are inferred from the claim text.