Probes utilizing universal tags, a kit comprising the same and detection methods

A multiple targets detection method of biomolecules,

• characterized in that the method comprises steps of:
• 1) preparing universal tags labeled with indicators, wherein the indicators are selected from the group consisting of fluorescent dyes, quantum dots, nanogolds, isotopes, biotins, and combinations thereof;
• 2) preparing probes;
• 3) linking the probes to a modified solid phase support to form probe arrays;
• 4) dissolving the universal tags and samples to be tested into a hybridization solution to hybridize with the probe arrays;
• or hybridizing the samples to be tested with the probes first, then hybridizing the universal tags with the probe arrays after rinsing;
• 5) rinsing to remove the redundant samples and the redundant universal tags;
• and
• 6) detecting the presence or absence of the indicators on the probe arrays with a fluorescence microscope, a flat scanner, or an array scanner, wherein:
• the biomolecules are selected from the group of molecules consisting of DNA, RNA, protein and/or saccharide;
• the universal tag is a fragment of DNA, RNA, peptide nucleic acid, or LNA, and is 3-20 mer in length;
• the probe contains in order from its 3′ terminus to 5′ terminus, a nucleotide sequence which is reverse complementary to a target molecule or a portion of a target molecule, and a nucleotide sequence which is reverse complementary to the universal tag, and a fragment of poly (T) or poly (A) optionally added on the 3′ terminus;
• or the probe contains in order from its 3′ terminus to 5′ terminus, a nucleotide sequence which is reverse complementary to the universal tag, a nucleotide sequence which is reverse complementary to a target molecule or a portion of a target molecule, and a fragment of poly (T) or poly (A) optionally added on the 5′ terminus.