Compositions and assays to detect swine h1n1 influenza a virus, seasonal h1 influenza a virus and seasonal h3 influenza a virus nucleic acids
AssigneeGen-Probe Incorporated
InventorEjan Tyler
Life of this patent
Estimating the term…
Abstract
Methods for detecting the presence or absence of the swine H1N1 influenza A virus, seasonal H1 influenza A virus and/or seasonal H3 influenza A virus nucleic acids in biological samples are disclosed. Compositions that are target-specific nucleic acid sequences and kits comprising target-specific nucleic acid oligomers for amplifying in vitro the swine H1N1 influenza A virus, seasonal H1 influenza A virus and/or seasonal H3 influenza A virus nucleic acid and detecting amplified nucleic acid sequences are disclosed.
Claims33
1-11. (canceled)
A composition comprising at least two seasonal H1 influenza A-specific amplification primers, wherein the primers comprise a nucleic acid oligonucleotide primer having at least 18 contiguous nucleotides encoding the H1 protein of H1 influenza A, or their completely complementary sequences, or DNA equivalents thereof.
17-21. (canceled)
23-28. (canceled)
A composition for detecting the presence of seasonal H1 influenza virus A comprising at least two oligonucleotides specific for sequences that represent a H1 influenza virus A genome or transcripts made from the H1 influenza virus A genome, selected from the group consisting of:
- SEQ ID NO:
- 63
- AGGTTTGTTTGGAGCCATTG;
- SEQ ID NO:
- 64
- TTGTTGAATTCTTTGCCCAC;
- SEQ ID NO:
- 65
- TCATTGAAGGGGGGTGGACTGGAA;
- SEQ ID NO:
- 66
- TGGACTGGAATGGTAGATGGTTGGT;
- SEQ ID NO:
- 67
- TCATTTTCTCAATTACAGAATTCACCTTGTTTG;
- SEQ ID NO:
- 68
- ATCATACAGAAAATGCTTATGT;
- SEQ ID NO:
- 69
- MAGCAGAGTCCAGTAGTA;
- SEQ ID NO:
- 70
- TTCACATTATAGCAGAAGATTCACCCCAG;
- SEQ ID NO:
- 71
- ACCCCAGAAATAGCCAAAAGACCC;
- SEQ ID NO:
- 72
- TTGAGGCAAATGGAAATCTAATA;
- SEQ ID NO:
- 73
- TACATTCTGGAAAGGAAGACT;
- SEQ ID NO:
- 74
- AGTAGAGGCTTTGGATCAGGAATCATC;
- SEQ ID NO:
- 75
- TGTTTATAGCTCCCTGAGGTGTTTGACA;
- and
- SEQ ID NO:
- 76
- CATTGGTGCATTTGAGGTGATGATTCCT.
32-34. (canceled)
(canceled)
40-44. (canceled)
A method for the detection of an influenza A virus from a sample, comprising the steps of:
- a. contacting an influenza A virus nucleic acid from a sample with a composition according to one of Mixture 1 to Mixture 23;
- b. providing conditions for amplifying the nucleic acid from step a by a polymerase chain reaction to generate an amplification product from the nucleic acid;
- and
- c. detecting the presence or absence of amplification product from step b, wherein the presence of the amplification product indicates that the sample contained an influenza A virus.
(canceled)
49-62. (canceled)
A method for the detection of a seasonal H1 Influenza A Virus from a sample, comprising the steps of:
- a. contacting a seasonal H1 Influenza A Virus nucleic acid from a sample with primer pair selected from Table 2;
- b. providing conditions for amplifying the nucleic acid from step a by a polymerase chain reaction to generate an amplification product from the nucleic acid;
- and
- c. detecting the presence or absence of amplification product from step b, wherein the presence of the amplification product indicates that the sample contained a seasonal H1 Influenza A Virus.
(canceled)
71-82. (canceled)
Dependencies are inferred from the claim text.