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US 2013/0296180 A1Published application
Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
AssigneeSequenom, Inc.
InventorsMathias EHRICH, Anders Clof Herman Nygren
Life of this patent
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Abstract
Provided are compositions and processes that utilize genomic regions differentially methylated between a mother and her fetus to separate, isolate or enrich fetal nucleic acid from a maternal sample. The compositions and processes described herein are useful for non-invasive prenatal diagnostics, including the detection of chromosomal aneuploidies.
Claims17
A method for enriching for fetal nucleic acid, comprising
- a) digesting nucleic acid from a pregnant female, which nucleic acid comprises fetal nucleic acid and maternal nucleic acid, with a methylation sensitive restriction enzyme that specifically digests the fetal nucleic acid at one or more loci that are hypomethylated in fetal nucleic acid;
- and
- b) separating the fetal nucleic acid from the maternal nucleic acid, thereby enriching for fetal nucleic acid.
Dependencies are inferred from the claim text.