Chemically-defined arrays for screening cell-substrate interactions
AssigneeWisconsin Alumni Research Foundation
InventorsJustin T. Koepsel, William L. Murphy
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Abstract
Patterned SAM arrays and methods of preparing patterned SAM arrays are disclosed. Advantageously, the methods used to prepare the patterned SAM arrays allow for controlling SAM spot-to-spot conditions such as ligand identity and ligand density, which allows for preparing a wide range of SAM spots in a single array format. Additionally, the patterned SAM arrays of the present disclosure support the culture of a range of cell types. The patterned SAM arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.
Claims20
A method for preparing a self-assembled monolayer array comprising:
- adhering a polymer stencil to a metal-coated substrate, wherein the polymer stencil comprises at least one well;
- forming at least one alkanethiolate self-assembled monolayer spot on the metal-coated substrate, wherein the alkanethiolate self-assembled monolayer spot is formed in the at least one well of the polymer stencil;
- removing the polymer stencil from the metal-coated substrate;
- and
- backfilling a region on the metal-coated substrate that surrounds the alkanethiolate self-assembled monolayer spot, wherein the backfilling forms an alkanethiolate self-assembled monolayer surrounding the at least one alkanethiolate self-assembled monolayer spot.
A self-assembled monolayer array comprising:
- at least one alkanethiolate self-assembled monolayer spot;
- and
- a self-assembled monolayer surrounding the at least one alkanethiolate self-assembled monolayer spot, wherein the self-assembled monolayer surrounding the at least one alkanethiolate self-assembled monolayer spot comprises a ligand.
A method of screening a cell-surface interaction comprising:
- preparing a self-assembled monolayer array, wherein the self-assembled monolayer array is prepared by
- adhering a polymer stencil to a metal-coated substrate, wherein the polymer stencil comprises at least one well;
- forming at least one alkanethiolate self-assembled monolayer spot on the substrate, wherein the alkanethiolate self-assembled monolayer spot is formed in the at least one well of the polymer stencil;
- removing the polymer stencil from the metal-coated substrate;
- and
- backfilling a region on the metal-coated substrate that surrounds the at least one alkanethiolate self-assembled monolayer spot, wherein the backfilling forms a self-assembled monolayer surrounding the at least one alkanethiolate self-assembled monolayer spot;
- contacting the cell with the self-assembled monolayer array;
- culturing the cell on the self-assembled monolayer array;
- and
- analyzing the cell.
Dependencies are inferred from the claim text.