Methods and Compositions for Analysis of Nucleic Acids
AssigneeAffymetrix, Inc.
InventorsGlenn K. Fu, Robert G. Kuimelis, Ronald J. Sapolsky
Life of this patent
Estimating the term…
Abstract
Compositions and methods for analysis of nucleic acids are disclosed. Targets are hybridized to arrays having features that include pairs of co-localized probes within features. The probe pairs may include a first probe type that is oriented so that the 5′ end is free and the 3′ end is attached to the support and a second probe type that is oriented so that the 3′ end is free for extension and the 5′ end is attached to the support. The probes of a feature are complementary to different regions of the same target sequence so they can simultaneously hybridize to a single target with a gap or nick between. The gap may be filled by extension and ligation or ligation.
Claims14
-20. (canceled)
A method for sequencing a target nucleic acid, the method comprising:
- hybridizing the target nucleic acid to a support bound probe, wherein the support bound probe is attached to a solid support, wherein the support bound probe comprises a first probe region and a second probe region, wherein the first probe region comprises a 5′ end and the second probe region comprises a 3′ end, and wherein both the first and second probe regions hybridize to the target nucleic acid;
- extending the 3′ end of the second probe region using the hybridized target nucleic acid as a template;
- circularizing the support bound probe to create a circularized probe, wherein circularizing comprises ligating the 5′ end of the first probe region with the 3′ end of the second probe region;
- digesting support bound probes that have not formed circularized probes;
- amplifying the circularized probe to create amplified circularized probes;
- and
- sequencing the amplified circularized probes.
The method of 21, wherein the solid support further comprises a plurality of sequencing primers.
Dependencies are inferred from the claim text.