Method for detection of basic peptide and reagent for detection of basic peptide
AssigneesKagoshima University, Sysmex Corporation
InventorsHiroyuki KABATA, Hideki TAKAHASHI, Rena TSURUOKA, Ikuro MARUYAMA
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Abstract
The present invention provides a method for detection of a basic peptide by mixing a sample suspected to contain the basic peptide and a reagent containing denatured albumin and detecting turbidness due to a complex of the basic peptide and denatured albumin.
Claims21
1-10. (canceled)
A method for diagnosing a disease comprising:
- obtaining a sample from a biological specimen suspected of having a disease, wherein said disease is identifiable by detecting a basic peptide;
- mixing the sample and a reagent comprising a heat-denatured albumin;
- forming a complex comprising the basic peptide and the heat-denatured albumin;
- obtaining optical information by irradiating the mixed sample and reagent with light;
- detecting the basic peptide using said optical information.
A method for identifying a disease within a biological specimen comprising:
- obtaining a sample from the biological specimen suspected of being diseased, wherein said disease is identifiable by detecting a basic peptide;
- mixing the sample and a reagent comprising a heat-denatured albumin;
- forming a complex comprising the basic peptide and the heat-denatured albumin;
- obtaining optical information by irradiating the mixed sample and reagent with light;
- detecting the basic peptide using said optical information;
- and
- identifying the disease.
A method for preparing a complex comprising a basic peptide and a heat-denatured albumin wherein said basic peptide is indicative of disease, comprising:
- obtaining a sample from a biological specimen suspected of having a disease, wherein said disease is identifiable by detecting a basic peptide;
- mixing the sample and a reagent comprising a heat-denatured albumin;
- forming a complex comprising the basic peptide and the heat-denatured albumin;
- obtaining optical information by irradiating the mixed sample and reagent with light;
- detecting the basic peptide using said optical information.
Dependencies are inferred from the claim text.