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US 2013/0295686 A1Published application
Intercalation methods and devices
AssigneeToxic Report LLC
InventorsRobert H. Meltzer, Joshua W. Griffis
Life of this patent
Estimating the term…
Abstract
The invention provides devices and methods for improving labeling of nucleic acids including intercalation of nucleic acids using for example mono intercalators such as mono cyanine intercalators.
Claims24
A method for uniformly labeling a nucleic acid with an intercalator comprising
- providing a capillary coupled to a microfluidic device, wherein the capillary comprises an intercalator,
- placing a first end of the capillary in a vessel comprising a nucleic acid,
- applying hydrodynamic or electrokinetic force sufficient to move the nucleic acid from the vessel through the capillary and to the microfluidic device,
- wherein flow of nucleic acid through the capillary is laminar, and wherein the intercalator is a mono intercalator.
1 more element
A system comprising
- a microfluidic device coupled to one end of a capillary comprising an intercalator that is a mono intercalator.
17-24. (canceled)
A method for uniformly labeling a nucleic acid with an intercalator comprising
- providing a microfluidic device comprising a sample inlet, a sheath fluid inlet, an elongation region, and a waste reservoir downstream of the elongation region,
- introducing a nucleic acid into the microfluidic device through the sample inlet,
- introducing intercalator into the microfluidic device through the sheath fluid inlet, and
- applying hydrodynamic force sufficient to move the nucleic acid from the sample inlet and the intercalator from the sheath fluid inlet through the elongation region to the waste reservoir,
- wherein flow of the nucleic acid and intercalator is laminar, and wherein the intercalator is a mono intercalator.
2 more elements
26-36. (canceled)
A microfluidic device comprising
- a sample inlet port, a sheath inlet port, an elongation region, and a waste reservoir downstream of the elongation region, wherein the sheath inlet port and the elongation region comprise an intercalator prior to introduction of a nucleic acid sample, wherein the intercalator is a mono intercalator.
(canceled)
A method for uniformly labeling a nucleic acid with an intercalator comprising
- providing a microfluidic chip comprising a sample inlet, an intercalator inlet, two sheath fluid inlets, an elongation region, and a waste reservoir downstream of the elongation region, wherein the intercalator inlet comprises two channels (“intercalator channels”) extending therefrom and merging with a channel extending from the sample inlet (“sample channel”) wherein the intercalator channels are positioned on opposite sides of the sample channel;
- and the two sheath fluid inlets are positioned on opposite sides of, and feed into, the elongation region
- introducing a nucleic acid into the microfluidic chip through the sample inlet,
- introducing intercalator into the microfluidic chip through the intercalator inlet, and
- applying hydrodynamic force sufficient to move the nucleic acid from the sample inlet and the intercalator from the intercalator inlet through the elongation region to the waste reservoir,
- wherein flow of the nucleic acid and intercalator is laminar, wherein the intercalator is a mono intercalator.
3 more elements
40-41. (canceled)
A microfluidic device comprising
- a sample inlet, an intercalator inlet, two sheath fluid inlets, an elongation region, and a waste reservoir downstream of the elongation region,
- wherein the intercalator inlet comprises two channels (“intercalator channels”) extending therefrom and merging with, and positioned on opposite sides of, a channel extending from the sample inlet (“sample channel”), and the two sheath fluid inlets are positioned on opposite sides of, and feed into, the elongation region.
Dependencies are inferred from the claim text.