Systems and methods for processing cells
AssigneeAuxocell Laboratories, Inc.
InventorsRouzbeh R. Taghizadeh, John Meade
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Abstract
The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.
Claims30
A tissue mincing tool comprising:
- a compartment for a tissue sample;
- a cutting surface at one end of the compartment;
- and
- a sterile, sealed container,
- wherein the cutting surface separates the compartment from the sterile, sealed container, such that a tissue sample that passes through the cutting surface is deposited within the sterile, sealed container.
A cell collection method comprising sedimenting cells in a cell collection device, wherein the cell collection device comprises:
- a sterile container for a fluid comprising cells;
- and
- a fluid passageway in communication with the sterile container, the fluid passageway comprising a cell collection area, wherein the volume of the cell collection area is less than 5% of the volume of the sterile container and the sterile container and the cell collection area are configured such that sedimentation of the cells from the fluid in the sterile container concentrates the cells in the cell collection area.
Dependencies are inferred from the claim text.