Method for producing nk cell-enriched blood preparation
AssigneeBiotherapy Institute of Japan
InventorsXuewen Deng, Hiroshi Terunuma, Mie Nieda
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Abstract
It is intended to provide a method for producing an NK cell-enriched blood preparation, which is low invasive and is capable of conveniently and rapidly growing NK cells, etc. in blood collected from an organism. The NK cells in blood are stimulated with NK cell growth-stimulating factors comprising an anti-CD16 antibody, OK432, an anti-CD3 antibody, and a cytokine. Then, the blood is cultured at a physiological cell temperature to produce an NK cell-enriched blood preparation.
Claims16
A method for producing an NK cell-enriched blood preparation, comprising:
- a stimulation step of stimulating NK cells comprised in blood collected from an organism, with NK cell growth-stimulating factors comprising at least an anti-CD 16 antibody, OK432, an anti-CD3 antibody, and a cytokine;
- and
- a culture step of culturing the blood at a physiological cell temperature after the stimulation step.
A composition for NK cell enrichment comprising an anti-CD 16 antibody, OK432, an anti-CD3 antibody, and a cytokine.
Dependencies are inferred from the claim text.