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US 2013/0295652 A1Published application
System and method for isolation of samples
AssigneeThe United States of America as represented by the Administrator of the National Aeronautics and Space Administration
InventorsYe Zhang, Honglu Wu
Life of this patent
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Abstract
Systems and methods for isolating samples are provided. The system comprises a first membrane and a second membrane disposed within an enclosure. First and second reservoirs can also be disposed within the enclosure and adapted to contain one or more reagents therein. A first valve can be disposed within the enclosure and in fluid communication with the first reservoir, the second reservoir, or both. The first valve can also be in fluid communication with the first or second membranes or both. The first valve can be adapted to selectively regulate the flow of the reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir.
Claims21
A sample isolation system, comprising:
- an air-tight enclosure;
- first and second membranes disposed within the enclosure;
- first, second, third and fourth reservoirs disposed within the enclosure, wherein each reservoir is adapted to contain one or more reagents therein;
- a first multi-way valve disposed within the enclosure and in fluid communication with the first or second or third reservoirs, and in fluid communication with the first or second membranes or both, wherein the first multi-way valve is configured for providing a first bidirectional flowpath between the first reservoir and the second reservoir and for providing a second bidirectional flowpath between the first reservoir and the third reservoir, wherein the first multi-way valve is adapted to selectively regulate flow of one or more reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir;
- and
- a second valve disposed within the enclosure and in fluid communication with the third and fourth reservoirs and the first multi-way valve, and in fluid communication with the first or second membranes or both, wherein the second valve is adapted to selectively regulate flow of one or more reagents from the third reservoir, through at least one of the first and second membranes, and into the fourth reservoir.
3 more elements
A sample isolation system, comprising:
- an air-tight enclosure;
- a first membrane support assembly having a first membrane disposed therein;
- a second membrane support assembly having a second membrane disposed therein;
- a first valve disposed between and operatively connected with the first and second membrane support assemblies;
- a second valve operatively connected with the second membrane support assembly;
- a first injector operatively connected with the first membrane support assembly, wherein the first injector comprises a first reservoir, and wherein the first reservoir is in fluid communication with the first valve through the first membrane;
- and
- a second injector operatively connected with the second valve, wherein the second injector comprises a second reservoir, and wherein the second reservoir is in fluid communication with the second membrane through the second valve.
5 more elements
A method of isolating nucleic acids from a sample, comprising:
- placing the sample into a sample isolation system;
- flowing a first reagent from a first reservoir through a first membrane and a first valve and into a second reservoir containing a second reagent to form a first mixture including the first and second reagents;
- and
- flowing the first mixture through the first valve, a second valve, and a second membrane and into a third reservoir.
1 more element
A sample isolation system, comprising:
- an air-tight enclosure;
- first and second membranes disposed within the air-tight enclosure;
- a first injector comprising a first reservoir;
- a second injector comprising a second reservoir;
- a third injector comprising a third reservoir;
- a fourth injector comprising a fourth reservoir;
- a fifth injector comprising a fifth reservoir;
- wherein the first, second, third, fourth, and fifth reservoirs are disposed within the enclosure, and wherein each reservoir is adapted to contain one or more reagents therein;
- a first multi-way valve disposed within the enclosure and positioned in fluid communication with the first reservoir and the second reservoir and the third reservoir and the fourth reservoir and in fluid communication with the first membrane and the second membrane, wherein the first multi-way valve is configured for providing a first bidirectional flowpath between the first reservoir and the second reservoir and for providing a second bidirectional flowpath between the first reservoir and the third reservoir, wherein the first multi-way valve is adapted to selectively regulate flow of one or more reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir;
- and
- a second multi-way valve disposed within the enclosure and positioned in fluid communication with the third reservoir and the fourth reservoir and the fifth reservoir and in fluid communication with the first multi-way valve and the second membrane, wherein the second multi-way valve is configured for providing a third bidirectional flowpath between the third reservoir and the fourth reservoir and for providing a fourth bidirectional flowpath between the fourth reservoir and the fifth reservoir, wherein the second multi-way valve is adapted to selectively regulate flow of one or more reagents from the third reservoir, through at least one of the first multi-way valve and the second membrane, and into the fourth reservoir.
8 more elements
Dependencies are inferred from the claim text.