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US 2013/0295644 A1Published application
Polypeptides Having Succinyl-CoA: Acetoacetate Transferase Activity and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorsBjarke Christensen, Peter Bjarke Olsen, Torsten Bak Regueira, Steen Troels Joergensen, Alan Berry
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Abstract
The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.
Claims20
An isolated protein complex having succinyl-CoA:acetoacetate transferase activity comprising a first polypeptide subunit and a second polypeptide subunit,
- wherein the first polypeptide subunit is selected from:
- (a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 3;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium-high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
- 1 or 2, or the full-length complementary strand thereof;
- and
- (c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or 2;
- and
- wherein the second polypeptide subunit is selected from:
- (a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 6;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium-high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO:
- 4 or 5, or the full-length complementary strand thereof;
- and
- (c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 4 or 5.
14 more elements
Dependencies are inferred from the claim text.