Compositions and methods of pgl for the increased production of isoprene
AssigneeDanisco US Inc.
InventorsZachary Q. BECK, Marguerite A. CERVIN, Alex T. NIELSEN, Caroline M. PERES
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Abstract
Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
Claims30
A method of producing isoprene, the method comprising:
- (a) culturing a composition comprising a recombinant cell of an Escherichia coli (E. coli) strain, or progeny thereof, under suitable culture conditions for the production of isoprene, wherein the cell comprises (i) one or more copies of a heterologous nucleic acid(s) encoding a 6-phosphogluconolactonase (PGL) polypeptide, wherein the nucleic acid is integrated in the E. coli chromosome and (ii) one or more heterologous nucleic acid(s) encoding isoprene synthase,
- wherein prior to the integration, the E. coli does not contain nucleic acids(s) encoding a PGL polypeptide, and wherein the resulting cell produces isoprene at a greater titer than that of the same cells that do not comprise (i);
- and
- (b) producing isoprene.
A composition comprising a recombinant cell of an Escherichia coli (E. coli) strain or progeny thereof, wherein the cell comprises (i) one or more copies of a heterologous nucleic acid(s) encoding a 6-phosphogluconolactonase (PGL) polypeptide wherein the nucleic acid is integrated in the E. coli chromosome and (ii) one or more heterologous nucleic acid(s) encoding isoprene synthase,
- wherein prior to the integration, the E. coli does not contain nucleic acids(s) encoding a PGL polypeptide, and wherein the resulting cell produces isoprene at a greater titer than that of the same cells that do not comprise (i).
Dependencies are inferred from the claim text.