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US 2013/0295609 A1Published application
Plant expression system
AssigneeMedicago Inc.
InventorsMarc-Andre D'Aoust, Pierre-Olivier Lavoie, Louis-Philippe Vezina
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AssigneeMedicago Inc.
InventorsMarc-Andre D'Aoust, Pierre-Olivier Lavoie, Louis-Philippe Vezina
Estimating the term…
A plant expression system and methods for expressing a protein of interest in a plant are provided. The plant expression system comprises a first nucleic acid sequence regulatory region sequence, operatively linked with a one or more than one comovirus enhancer, a nucleotide sequence of interest, one or more than one geminivirus amplification elements, and a second nucleic acid encoding a geminivirus replicase. The method of producing a protein of interest in a plant, involves introducing the plant expression system into a plant, or portion of the plant, and incubating the plant or the portion of the plant under conditions that permit the expression of the nucleotide sequence and producing the protein of interest.
1-44. (canceled)
A plant expression system comprising a first nucleic acid sequence comprising a regulatory region, operatively linked with a one or more than one comovirus enhancer, a nucleotide sequence encoding an influenza hemagglutinin wherein the influenza hemagglutinin is selected from influenza type B hemagglutinin, influenza type A subtype 113 hemagglutinin, and influenza type A subtype H5 hemagglutinin, and one or more than one geminivirus amplification element, and a second nucleic acid sequence encoding a geminivirus replicase.
A method of producing an influenza hemagglutinin or an influenza virus like particle (VLP) in a plant or in a portion of a plant, the method comprising introducing into the plant or in the portion of a plant the plant expression system of claim 1, and incubating the plant or the portion of a plant under conditions that permit expression of the nucleotide sequence encoding the influenza hemagglutinin, thereby producing the influenza hemagglutinin or the influenza VLP.
Dependencies are inferred from the claim text.