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US 2013/0288263 A1Published application
Device For Detection Of Target Molecules And Uses Thereof
AssigneeInvisible Sentinel, Inc.
InventorsNicholas A. Siciliano, Martin Joseph Bouliane
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Abstract
Devices and methods for the detection of target molecules are disclosed. Devices and methods for detecting food-borne target molecules are also disclosed.
Claims19
A device comprising a housing, the housing comprising a first housing member, a second housing member, and inlet opening,
- wherein the housing further comprises:
- a membrane detection system,
- wherein the membrane detection system comprises:
- a conjugate pad;
- a permeable membrane;
- a test membrane;
- and
- two absorbent members;
- a force member, wherein the vertical axis of the force member is substantially perpendicular to the membrane detection system;
- a slidable locking member contacting the force member;
- an attachment member in contact with the locking member;
- and
- a sliding button attached to the slidable locking member
- wherein the conjugate pad, the permeable membrane, the test membrane;
- and the two absorbent members are substantially parallel to each other and the inlet opening is in fluid contact with the membrane detection system.
12 more elements
A method of detecting a target molecule comprising:
- a) contacting a sample with a device, the device comprising a housing, the housing comprising a first housing member, a second housing member, and inlet opening,
- wherein the housing further comprises:
- a membrane detection system,
- wherein the membrane detection system comprises:
- a conjugate pad comprising a first capture reagent;
- a permeable membrane;
- a test membrane comprising a second capture reagent;
- and
- two absorbent members;
- a force member, wherein the vertical axis of the force member is substantially perpendicular to the membrane detection system;
- a slidable locking member contacting the force member;
- an attachment member in contact with the locking member;
- and
- a sliding button attached to the slidable locking member
- wherein the conjugate pad, the permeable membrane, the test membrane, and the two absorbent members are substantially parallel to each other and the inlet opening is in fluid contact with the membrane detection system.
- wherein the sample flows through the inlet opening and vertically flows from the conjugate pad to the test membrane;
- wherein the conjugate pad is operably connected to the slidable locking member and the conjugate pad is moved by moving the slidable locking member
- b) moving the conjugate pad after a portion of the sample has contacted and flowed through the conjugate pad, thereby exposing at least a portion of the test membrane for detection;
- and
- c) identifying a positive or negative reaction for the target molecule.
17 more elements
The method of claim 16, wherein the sample is contacted with the conjugate pad prior to compressing the membrane detection system with the force member.
The method of claim 16, wherein the target molecule is a polynucleotide.
Dependencies are inferred from the claim text.