# US 2013/0280765 A1 · Method for Transforming Iota-Carrageenan Into Alpha-Carrageenan by Means of a New Class of 4S-Iota-Carrageenan Sulfatase

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- Number: US 2013/0280765 A1
- Document ID: US20130280765A1
- Status: Published application
- Kind: A1
- Application number: 13/879,129
- Filed: 2011-10-17
- Published: 2013-10-24
- Earliest claimed: 2010-10-15
- Assignees: Centre National de la Recherche Scientifique (CNRS)
- Inventors: William Helbert; Aurelie Prechoux; Sabine Genicot-Joncour
- Web page: https://patentgrep.com/patent/US20130280765A1

## CPC

- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12N 9/16: acting on ester bonds (3.1)
- C12Y 301/06: Sulfuric ester hydrolases (3.1.6)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

A method for transforming iota-carrageenan into alpha-carrageenan by a new class of 4S-iota-carrageenan sulfatase. The invention also relates to carrageenans obtained by the conversion method. The invention can be especially applied to the agro-food, pharmaceutical and cosmetic industries.

## Claims

### Claim at position 1

1-4. (canceled)

### Claim 5

A method for transforming iota-carrageenan to alpha-carrageenan comprising the enzymatic catalysis of a conversion of an iota-carrabiose motif to an alpha-carrabiose motif by an enzyme whose peptide sequence comprises the peptides having the following sequences:
(SEQ ID NO: 1)
NGQFDNTVIVFTSDNGGK,
(SEQ ID NO: 2)
FDQTFQVGDNTR,
and
(SEQ ID NO: 3)
ETEYITDGLSR.

### Claim 6 (depends on 5)

The method as claimed in claim 5, in which the peptide sequence of the enzyme is
(SEQ ID NO: 4)
MTFNKKVSTLLWGTLIAISVGNASAADAGQSKADESNEKPNILFVLA
DDLGYNDVGFNGSTDIKTPNLDGLAKNGMTFDAAYVAHPFCGPSRAA
IMTGRYPHKIGAQFNLPEDNSNVGVSADELFIAQTMKSAGYFTGAMG
KWHLGEASEYHPNKHGFDEFYGFLGGGHNYFPEQFEAAYNKRVAQGM
TNINMYLTPLEHNGKEVRETEYITDGLSREAVNFVDKAAAKKKPFFL
YLAYNAPHVPLQAKEEDMAMFSQIKDKKRRTYAGMVYAVDRGVGRIV
EQLKKNGQFDNTVIVFTSDNGGKLGQGANNYPLKEGKGSVQEGGFRT
PMLVHWPKHMKAGSRFSHPVLALDLYPTFAGLGGAVLPEDKKLDGKD
IWADIQANTAPHKDEFIYVLRHRNGYSDAAARRNQFKAVKNHNDDWK
LYNIAQDISEDNDISAQHPDILRDMVSSMESWSWNNQQPKWFHQSAE
GAQWRLKAMPRFDQTFQVGDNTRSNSKKGH.

### Claim 7 (depends on 5)

The method as claimed in either of claim 5, in which said enzyme is a 4S-iota-carrageenan sulfatase.

### Claim 8 (depends on 5)

The method as claimed in claim 5, in which the enzyme is produced by a host cell comprising a nucleic acid encoding said enzyme and/or a vector comprising a nucleic acid sequence encoding said enzyme.