Aspergillus containing beta-glucosidase, beta-glucosidases and nucleic acids encoding the same
AssigneeCleanvantage LLC
InventorsPhilip Teller, Peter S. Lubeck, Annette Sorensen, Birgitte K. Ahring
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Abstract
A novel microorganism is provided named Aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material. Also, host organisms comprising the polypeptides of the invention and/or polynucleotides encoding these are provided. In addition, methods, compositions, and kit-of-parts are provided which comprise any component of the invention, and optionally any additional components.
Claims29
An isolated microorganism of the species Aspergillus saccharolyticus.
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An isolated polypeptide having beta-glucosidase activity comprising:
- a. an amino acid sequence selected from SEQ NO:
- 3 and 4,
- b. a biologically active sequence variant of any of SEQ NO:
- 3 and 4, wherein said variant has at least 92% sequence identity to said SEQ NO:
- 3 or 4, or
- c. a biologically active fragment of at least 30 consecutive amino acids of any of the amino acid sequences of a) through b.
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An isolated polynucleotide comprising a nucleic acid or its complementary sequence being selected from the group consisting of:
- a. a polynucleotide sequence encoding a polypeptide consisting of an amino acid sequence SEQ ID NO:
- 3 and 4,
- b. a polynucleotide sequence encoding a biologicaly active sequence variant of said amino acid sequence, wherein the variant has at least 92% sequence identity to said SEQ ID NO:
- 3 and 4, and
- c. a polynucleotide sequence encoding a biologically active fragment of at least 30 consecutive amino acids of any of the amino acid sequences of a) through b), or
- d. SEQ ID NO.:
- 1, 2 or 29, or
- e. a polynucleotide comprising a nucleic acid sequence having at least 85% identity to SEQ ID NO:
- 1, 2 or 29, or
- f. a polynucleotide hybridising to SEQ ID NO.:
- 1, 2 or 29, and
- a polynucleotide complementary to any of a) to f).
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Dependencies are inferred from the claim text.