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US 2013/0280754 A1Published application
3D Cell Viability Assay
AssigneeNano3D Biosciences, Inc.
InventorGlauco R. Souza
Life of this patent
Estimating the term…
Abstract
Cells are grown in 3D culture and topological features obtained by photomicrography are correlated to cell viability and cell cell interactions.
Claims20
A cell viability or cell-cell interaction assay comprising:
- a) culturing a 3D cell culture;
- b) taking photomicrographs of said 3D cell culture at one or more times;
- c) analyzing said photomicrographs to measure one or more of i) fractal dimension, ii) the number of cell clusters, iii) cell cluster size, iv) total area of cell clusters, or v) rate of wound closure;
- d) wherein fractal dimension, rate of wound closure, and cell cluster size are directly proportional to cell viability or cell-cell interactions, and the number of cell clusters, and total area of cell clusters are inversely proportional to cell viability or cell-cell interactions.
1 more element
A cell assay comprising:
- a) culturing a plurality of samples of levitated cells to form cell clusters, one or more of said plurality of samples being without a test agent, and one or more of said plurality of samples being with one or more concentrations of said test agent;
- b) taking photomicrographs of said plurality of samples of levitating cells at one or more times;
- c) analyzing said photomicrographs to measure one or more of i) fractal dimension, ii) the number of cell clusters, iii) cell cluster size, iv) total area of cell clusters, or v) rate of wound closure;
- d) wherein fractal dimension and rate of wound closure and cell cluster size are directly proportional to cell viability or cell-cell interactions, and the number of cell clusters and total area of cell clusters are inversely proportional to cell viability or cell-cell interactions.
1 more element
A cell-cell interaction or viability assay comprising:
- a) culturing a plurality of samples of 3D cell cultures, one or more of said plurality of samples being without a test agent, and one or more of said plurality of samples being with one or more concentrations of said test agent;
- b) taking photomicrographs of said plurality of samples of 3D cells cultures at one or more times;
- c) analyzing said photomicrographs to measure one or more of i) fractal dimension, ii) the number of cell clusters, iii) cell cluster size, iv) total area of cell clusters, or v) rate of wound closure,
- d) wherein fractal dimension and rate of wound closure and cell cluster size are directly proportional to cell-cell interaction and viability, and the number of cell clusters and total area of cell clusters are inversely proportional to cell-cell interaction and cell viability, and wherein a decrease in cell-cell interaction indicates that said test agent is inhibitory, and wherein an increase in cell-cell interactions indicates that said test agent is stimulatory;
- e) wherein said 3D cell cultures are prepared by levitating cells with a composition comprising:
- a) a negatively charged nanoparticle;
- b) a positively charged nanoparticle;
- and c) a support molecule, wherein one of said negatively charged nanoparticle or positively charged nanoparticle contains a magnetically responsive iron or iron oxide, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture.
5 more elements
Dependencies are inferred from the claim text.