# US 2013/0280423 A1 · Pipette tip having a hydrophobic surface texture

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- Number: US 2013/0280423 A1
- Document ID: US20130280423A1
- Status: Published application
- Kind: A1
- Application number: 13/921,668
- Filed: 2013-06-19
- Published: 2013-10-24
- Earliest claimed: 2010-07-12
- Assignees: Hamilton Bonaduz AG
- Applicants: Hamilton Bonaduz AG
- Inventors: Vinzenz KIRSTE; Renato Nay; Rainer Beckbissinger
- Web page: https://patentgrep.com/patent/US20130280423A1

## CPC

- B01L 3/0275: {Interchangeable or disposable dispensing tips}
- B01L 3/021: {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids}
- B01L 2200/12: Specific details about manufacturing devices
- B01L 2300/165: Specific details about hydrophobic, oleophobic surfaces
- B01L 2300/166: Suprahydrophobic; Ultraphobic; Lotus-effect
- B05C 17/00503: {Details of the outlet element (B05C17/00523 takes precedence; nozzles for deformable tubular containers B65D35/38)}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B05C: APPARATUS FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL (spraying apparatus, atomising apparatus, nozzles B05B; plant for applying liquids or other fluent materials to objects by electrostatic spraying B05B5/08)

## Abstract

The present invention relates to a pipette tip (10), for aspirating and dispensing pipetting fluid, which extends along a pipette tip longitudinal axis (L), a first axial longitudinal end region (16) of the pipette tip (10), as a pipetting longitudinal end region (16), comprising a pipette opening (12), through which pipetting fluid can flow in the course of operation, and a second axial longitudinal end region (18) of the pipette tip (10), as a coupling longitudinal end region (18), which opposes the pipetting longitudinal end region (16) in the axial direction, comprising a coupling shape, for coupling, preferably releasable coupling, to a coupling counter-shape of a pipette device, the pipette tip (10) comprising an outer hydrophobic region (32) on the outside (30) thereof and an inner hydrophobic region (26) on the inside (28) thereof, each having a quadratic roughness in a range of 100 nm to 1000 nm, preferably of 150 nm to 750 nm and particularly preferably of 200 nm to 500 nm, and having a peak-to-peak roughness in a range of 800 nm to 5500 nm, preferably of 1750 nm to 4500 nm and particularly preferably of 2500 nm to 3700 nm, the axial extension range of the outer hydrophobic region (32) and the axial extension range of the inner hydrophobic region (26) differing from one another.

## Claims

### Claim 1

Process for hydrophobically coating pipette tips, which comprises wetting at least regions of the inside of the pipette tip with a wetting solution, characterised in that it more precisely comprises the steps of:
providing a holding cavity other than the pipette tip, preferably a tube member, particularly preferably a glass tube member, on a pipette device,
immersing the holding cavity in the wetting solution,
aspirating wetting solution into the holding cavity,
connecting the pipette tip to the holding cavity,
dispensing the aspirated wetting solution from the holding cavity through the pipette tip and thus rinsing a pipetting fluid holding chamber portion inside the pipette tip,
evaporating solvent contained in the wetting solution.

### Claim 2 (depends on 1)

Process according to claim 1, characterised in that the evaporating step comprises heating the pipette tip and/or passing a fluid through the pipette tip, preferably a gas, particularly preferably air, in particular dry air.

### Claim 3 (depends on 2)

Process according to claim 2, characterised in that it comprises providing the wetting solution at a temperature in a range of 65° C. to 85° C., preferably in a range of 70° C. to 80° C., particularly preferably at approximately 75° C.

### Claim 4 (depends on 3)

Process according to claim 3, characterised in that the wetting solution comprises a polymer or copolymer, preferably a polypropylene-polyethylene copolymer, and a preferably xylol-based solvent which dissolves this.

### Claim 5 (depends on 1)

Process according to claim 1, characterised in that the wetting solution is dispensed through the pipette tip at a flow rate of 0.3 ml/s to 0.7 ml/s, preferably of 0.4 ml/s to 0.6 ml/s, and/or in that the wetting solution is dispensed through the pipette tip at a temperature of 20° C. to 30° C., preferably of 21° C. to 25° C., particularly preferably of 22° C. to 25° C.