1-deoxy-d-xylulose 5-phosphate synthase alleles responsible for enhanced terpene biosynthesis
AssigneeGenoplante-Valor
InventorsPhlippe Hugueney, Eric Duchene, Didier Merdinoglu
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Abstract
A method of enhancement of the 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity of plants or bacteria to increase terpenes production in cells, an enhanced DXS sequence likely to be obtained by this method, a method of enhancement of production of terpenes in a host cell containing the enhanced DXS enzyme, and transgenic bacterium or plants that express this polypeptide are described.
Claims22
1-21. (canceled)
A method of enhancement of the 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity of plants or bacteria to increase terpenes production comprising the steps of:
- a) Determining at least one mutation site, preferably two, on the polypeptide having a DXS activity of a plant, a bacteria or a yeast by doing a sequence comparison between a reference sequence selected in the group comprising SEQ ID No2, SEQ ID No4, SEQ ID No6, SEQ ID No19, SEQ ID No20, SEQ ID No21, SEQ ID No22 and SEQ ID No23 and the sequence of said plant, bacteria or yeast;
- b) Proceeding to the mutation on at least one mutation site, preferably on two mutation sites;
- c) Obtaining a polypeptide having an enhanced DXS activity compared to the typical DXS.
A polypeptide of plant or bacteria having an enhanced DXS activity compared to the typical DXS of said plant or bacteria.
Dependencies are inferred from the claim text.