Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
AssigneeAdvanced Cell Technology, Inc.
InventorMichael West
Life of this patent
Estimating the term…
Abstract
Methods for producing stem cell banks, preferably human, which optionally may be transgenic, e.g., comprised of homozygous MHC allele cell lines are provided. These cells are produced preferably from parthenogenic, IVF, or same-species or cross-species nuclear transfer embryos or by dedifferentiation of somatic cells by cytoplasm transfer. Methods for using these stem cell banks for producing stem and differentiated cells for therapy, especially acute therapies, and for screening for drugs for disease treatment are also provided.
Claims133
A stem cell bank comprising a library or plurality of human or non-human animal stem cell lines, each of which is homozygous for at least one MI-IC allele present in a human or non-human animal population, wherein which each member of said plurality of stem cell lines is homozygous for a different set of MHC alleles relative to the remaining members of the plurality of stem cell lines.
A method for treatment, preferably acute treatment, comprising transplanting cells or tissue that are homozygous for at least HLA one allele in a person in need of such a transplant, comprising:
- a. identifying the MHC alleles of a person in need of a transplant (the recipient);
- b. obtaining from a stem cell bank comprising a plurality of stem cells homozygous for at least one MHC allele of the transplant recipient;
- c. generating cells or tissue suitable for transplant from said stem cells;
- and
- d. transplanting said cells or tissue suitable for transplant into said recipient.
A method for producing stem cells homozygous for at least one MHC allele present in a human population, comprising:
- a) obtaining oocytes from a donor;
- b) activating the oocytes;
- c) exposing the activated oocytes to chemical treatment that inhibits extrusion of the second polar body;
- d) culturing the embryo in vitro under conditions resulting in formation of a blastocyst;
- e) culturing inner cell mass cells of the blastocyst in vitro under conditions resulting in production of stem cells;
- and
- f) screening to identify stem cells homozygous in a particular MHC allele.
A diploid cell line that is parthenogenetically-derived and is homozygous for at least one recessive disease-causing allele.
A method for producing stem cells homozygous for at least one recessive disease-causing allele, comprising:
- a) obtaining oocytes from a donor whose DNA contains a recessive disease-causing allele;
- b) activating the oocytes;
- exposing the activated oocytes to chemical treatment that inhibits extrusion of the second polar body;
- c) culturing the embryo in vitro under conditions resulting in formation of a blastocyst;
- d) culturing inner cell mass cells of the blastocyst in vitro under conditions resulting in production of stem cells;
- and
- f) screening the stem cells to identify stem cells that are homozygous for a recessive disease-causing allele.
Dependencies are inferred from the claim text.