Coriolus versicolor extracts, methods of isolating biologically-active compounds, and uses thereof
AssigneesVersitech Limited, Purapharm Company Limited
InventorsALLAN SIK YIN LAU, LAI HUNG CINDY YANG, STANLEY CHI CHUNG CHIK
Life of this patent
Estimating the term…
Abstract
The subject invention provides efficient and convenient methods of isolating 9-oxo-10E, 12E-octadecadienoic acid methyl ester (9-KODE methyl ester) from C. versicolor. In addition, the subject invention provides therapeutic uses of Coriolus versicolor extracts, biologically-active chemical substituents isolated from C. versicolor, as well as 9-KODE methyl ester and related compounds. In a preferred embodiment, the subject invention can be used to inhibit the metastatic spread of cancer cells.
Claims19
A method of obtaining isolated 9-oxo-10E, 12E-octadecadienoic acid methyl ester (9-KODE methyl ester), comprising the steps of:
- a) providing a sufficient quantity of raw material of Coriolus versicolor;
- and
- b) extracting the raw material of Coriolus versicolor with a polar solvent that is alcohol or an alcohol-water mixture at a temperature of about 15° C. to about 30° C. to yield a Coriolus versicolor alcohol extract and a residue, and recovering the Coriolus versicolor alcohol extract;
- and
- c) isolating 9-oxo-10E, 12E-octadecadienoic acid methyl ester from the Coriolus versicolor alcohol extract
- wherein step b) is performed once or more than once.
A compound having the chemical structure represented by formula I, or a salt thereof,
- wherein is a double bond or a single bond;
- R1 is H, OH, a straight or branched chain C1 to C4 alkyl group, or ORa wherein Ra is a straight or branched chain C1 to C4 alkyl group;
- and
- R2 is H, O, or a straight or branched chain C1 to C4 alkyl group;
- and
- R3 is H, OH, O, halo, a straight or branched chain C1 to C4 alkyl group, or ORa wherein Ra is a straight or branched chain C1 to C4 alkyl group.
A method for treating cancer or a tumor, comprising administering, to a subject in need of such treatment, an effective amount of a composition comprising a compound of formula I, or a salt thereof,
- wherein is a double bond or a single bond;
- R1 is H, OH, a straight or branched chain C1 to C4 alkyl group, or ORa wherein Ra is a straight or branched chain C1 to C4 alkyl group;
- and
- R2 is H, O, or a straight or branched chain C1 to C4 alkyl group;
- and
- R3 is H, OH, O, halo, a straight or branched chain C1 to C4 alkyl group, or ORa wherein Ra is a straight or branched chain C1 to C4 alkyl group.
Dependencies are inferred from the claim text.