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US 2013/0274145 A1Published application
Anti-TNF Alpha Fibronectin Type III Domain Based Scaffold Compositions, Methods and Uses
AssigneeCentorcor Ortho Biotech, Inc.
InventorsSteven Jacobs, Karyn O'Neil
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Abstract
A protein scaffold based on a consensus sequence of the tenth fibronectin type III (FN3) repeat from human fibronectin, preferably human Tenascin, that binds to human TNFα including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices.
Claims4
A method of constructing a library of a protein scaffold based on a fibronectin type III (FN3) domain capable of binding to human TNFα, comprising the steps of:
- providing a protein scaffold based on a fibronectin type III (FN3) domain comprising:
- a backbone portion comprising the amino acid sequence of residues 1-21, 29-74, and 82-89 of SEQ ID NO:16;
- a B:C loop portion between the backbone portion residues corresponding to residues 21 and 29 of SEQ ID NO:16, wherein the B:C loop portion has an amino acid sequence that has at least 75% identity to the amino acid sequence of SEQ ID NOS:22, 23, 36, 41, 42, 44, 45, 52, 58, 63, or 64;
- and
- an F:G loop portion between the backbone portion residues corresponding to residues 74 and 82 of SEQ ID NO:16, wherein the F:G loop portion has an amino acid sequence that has at least 75% identity to the amino acid sequence of SEQ ID NOS:78, 80, 96, 97, 112, 114, 122, 127, 145, 157, or 167;
- and
- introducing diversity into copies of the protein scaffold to form the protein scaffold library.
4 more elements
Any invention described herein.
Dependencies are inferred from the claim text.