Method and system for detecting a target within a population of molecules
AssigneeRamot at Tel-Aviv University Ltd.
InventorsAdy ARIE, Amos Danielli
Life of this patent
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Abstract
A method of detecting a target within a population of molecules comprising: contacting a plurality of labeled probe molecules with the population of molecules potentially containing a target of the probe molecules; acquiring a probe specific signal emitted by said labeled probe molecules that bound to said target together with a background signal; preferentially modulating said probe specific signal by at least one of modulating said acquisition and modulating an emission of said probe specific signal; and detecting said probe specific signal over said background signal using said preferential modulation.
Claims24
A method of detecting at least one target molecule within a population of molecules, the method comprising:
- contacting a plurality of labeled probe molecules, each selectively binds to at least one target molecule, with the population of molecules potentially containing a target of said at least one target molecule;
- directing an excitation beam through the plurality of probe molecules, thereby producing a background signal and potentially a probe specific signal emitted if at least one of said plurality of labeled probe molecules is bound to at least a portion of said target;
- detecting said probe specific signal over said background signal by performing a preferential modulation to at least one of said acquisition and an emission of said probe specific signal, wherein said detecting according to said probe specific signal includes detecting by moving the excitation beam relative to the target molecule, and;
- detecting at least one of the presence, the absence and the amount of said at least one target molecule in said target according to said probe specific signal.
A system for analysis of a population of molecules, comprising:
- a vessel adapted to contain a plurality of labeled probe molecules, each selectively binds to at least one target molecule, in contact with a population of molecules potentially containing a target comprising said at least one target molecule so that at least one of said plurality of labeled probe molecules being bound to at least a portion of said target;
- a fluorescent excitation source adapted to direct an excitation beam through the vessel, the beam configured to cause at least some of said at least one labeled probe molecule to emit a probe specific signal;
- a detector adapted to detect said probe specific signal and produce a detection output accordingly;
- a signal modulator configured to perform a preferential modulation to at least one of the probe specific signal and said detection output;
- and
- an analysis module adapted to analyze the detection output in consideration of said preferential modulation, wherein the detection includes detecting by analyzing a signal provided by the excitation beam when the excitation beam is moved relative to at least one bound probe molecule.
Dependencies are inferred from the claim text.