Advanced Reverse-phase Magnetic Immunoassay
OwnerJung-Rok Lee, Richard Gaster +1inventors
InventorsJung-Rok Lee, Richard Gaster, Shan X. Wang
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Abstract
A magnetic assay is provided where capture probes are disposed on the sensor array as capture probe spots that overlap with the sensor elements. The capture probes on the sensor elements can be the same or they can be different. Two or more sample solutions are also disposed on the sensor array as sample spots that overlap with the sensor elements. Targets in the sample solutions can bind to the capture probes to provide immobilized targets. Magnetically labeled probes capable of binding to targets are provided to the assay, and the resulting assay signal is from immobilized magnetically labeled probes at the sensor elements.
Claims15
A method for performing a biological or chemical assay, the method comprising:
- providing an array of magnetic sensors including two or more sensor elements;
- immobilizing capture probes on the sensor elements, wherein the immobilized capture probes are configured as discrete capture probe spots, each capture probe spot overlapping with a corresponding one of the sensor elements;
- providing sample solutions on the sensor elements, wherein the sample solutions comprise two or more distinct individual samples disposed on different sensor elements, wherein target species in the sample solutions may bind to immobilized capture probes to become immobilized targets;
- and wherein the sample solutions are configured as discrete sample spots, each sample spot overlapping with a corresponding one of the sensor elements;
- providing one or more magnetically labeled probes to the sensor elements capable of binding to the targets;
- and
- providing signal measurements from the sensor elements responsive to immobilized magnetically labeled probes as an assay output.
Dependencies are inferred from the claim text.