Stress tolerant bifidobacteria
AssigneeNestec S.A.
InventorsBernard Berger, Enea Rezzonico, Fabrizio Arigoni
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Abstract
The present invention relates in general to the field of Bifidobacteria. In particular, the present invention relates to the field of Bifidobacteria with a modulated stress resistance. One embodiment of the present invention is a Bifidobacterium with a constitutively modulated DnaK, GrpE and/or CIpB expression.
Claims15
A method for increasing the number of living probiotic bacteria in a product comprising the steps of producing the product using Bifidobacterium with a constitutively modulated expression of a gene selected from the group consisting of DnaK, DnaJ, GrpE, ClpB and combinations thereof, and the Bifidobacterium is selected from the group consisting of Bifidobacterium longum NCC2912, Bifidobacterium longum NCC2913, Bifidobacterium longum NCC2923 and combinations thereof.
A method to increase the stress resistance of a Bifidobacterium comprising the step of constitutively upregulating an expression of a gene selected from the group consisting of DnaK, DnaJ, GrpE, ClpB and combinations thereof, by introducing at least one point mutation in the hspR gene of the Bifidobacterium so that a characteristic selected from the group consisting of HspR expression, HspR functioning and a combination thereof is hindered.
Dependencies are inferred from the claim text.