Method to increase gene targeting frequency
AssigneeRegents of the University of Minnesota
InventorEric Hendrickson
Life of this patent
Estimating the term…
Abstract
Gene targeting is a valuable tool for basic re-searchers and gene therapists. Unfortunately, current methods utilized to target genes are inefficient because of their low targeting frequencies. Provided herein are methods and compositions by which gene targeting frequencies can be increased.
Claims22
A method to increase gene targeting frequency comprising inhibiting expression of at least one gene of a DNA double strand break (DSB) repair pathway or by inhibiting activity of at least one protein of a DNA DSB repair pathway so as to provide increased gene targeting frequency as compared to a cell in which expression and/or activity has not been inhibited.
A method to reduce stable random exogenous DNA integration comprising inhibiting expression of at least one gene of a DNA DSB repair pathway or by inhibiting activity of at least one protein of a DNA DSB repair pathway so as to provide decreased random DNA integration as compared to a cell in which expression and/or activity has not been inhibited.
A method to increase stable targeted DNA integration comprising inhibiting expression of at least one gene of a DNA DSB repair pathway or by inhibiting activity of at least one protein of a DNA DSB repair pathway so as to provide increased targeted DNA integration as compared to a cell in which expression and/or activity has not been inhibited.
Dependencies are inferred from the claim text.