Vectors and methods for recombinant protein expression
AssigneeAdv Biologics ,Inc.
InventorXiaoyun Wu
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Abstract
The present invention discloses a series of eukaryotic expression vectors utilizing the reduction of transcription read-through events to create stable and high-yield cell lines for recombinant protein expression. The vectors comprise more than one polyadenylation signal or one or more polyadenylation signals plus other DNA fragment which is known to enhance transcription termination to control the expression level of selection marker, with the configuration to transcribe the minimal level of full-length bicistronic mRNA to express the selection marker, which can be used to create stable cell lines at high expression levels, without the need for drug selection or drug mediated gene amplification.
Claims16
An eukaryotic expression vector comprising a first promoter operatively linked to a first coding sequence and an enhanced termination unit that comprises two or more polyadenylation signal sequence, followed by an internal ribosome entry site (IRES) and a second coding sequence.
Dependencies are inferred from the claim text.