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US 2013/0273638 A1Published application
Enhanced gene expression in algae
AssigneeSynaptic Research, LLC
InventorsGeorge Oyler, Julian Rosenberg
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Abstract
The invention provides eukaryotic unicellular algae engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.
Claims14
A eukaryotic unicellular algae, comprising:
- a construct for enhanced gene expression in an alga cell, wherein said construct further comprises
- a eukaryotic unicellular algae compatible transcriptional promoter functionally upstream of a coding sequence for a gene expression enhancer (GEE) fusion protein, wherein the fusion protein comprises an algae derived p300 functionally fused to the DNA binding protein, wherein at least the portion of the coding sequence of the DNA binding protein domain is codon optimized for improved expression in a eukaryotic unicellular algae;
- at least one transgene functionally downstream of a eukaryotic unicellular algae compatible transcriptional promoter;
- and
- at least one DNA region that is a binding site for the DNA binding protein, in vicinity of at least one of said transcriptional promoters.
2 more elements
Dependencies are inferred from the claim text.