Depletion of host cell components from live vector vaccines
AssigneeProbiogen AG
InventorsIngo Jordan, Holger Bernhardt, Stefan Hartmann
Life of this patent
Estimating the term…
Abstract
It is desirable to produce live vaccines, which are highly attenuated and which do only contain minimal or no animal-derived components. The production of highly attenuated live viruses can be better achieved when using specifically designed cell lines as producer substrate as opposed to using less defined primary cells. However, live viruses, thus produced comprise undesirable components from the cell lines and cell culture. The present invention relates to methods of production and purification of live enveloped viruses, which are suitable for vaccination.
Claims22
A method of virus purification comprising the steps of
- (i) adding one or more chaotropic salts and/or polar or charged macromolecules to a virus producing cell prior to cell lysis,
- (ii) lysing said virus producing cell, and
- (iii) separating said virus from at least part of the non-viral substances comprised in said virus producing cell or its cell culture medium.
3-4. (canceled)
9-11. (canceled)
(canceled)
A method for virus purification comprising the steps of
- (i) lysing a virus producing cell, and
- (ii) applying the lysate of said virus producing cell onto a siliceous substance.
Dependencies are inferred from the claim text.