Novel method of production of 2,4-dihydroxybutic acid
AssigneeAdisseo France S.A.S.
InventorsThomas Walther, Hélène Cordier, Christopher Topham, Isabelle Andre, Magali Remaud-Simeon, Robert Huet, Jean-Marie Francois
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Abstract
The present invention deals with a method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway comprising the transformation of malate in 4-phospho-malate using a malate kinase, said 4-phospho-malate being transformed in malate-4-semialdehyde using a malate semialdehyde dehydrogenase and said malate-4-semialdehyde being transformed in 2,4-DHB using a DHB dehydrogenase.
Claims56
Method of producing 2,4-dihydroxybutyric acid (2,4-DHB) comprising:
- a first step of transforming malate into 4-phospho-malate using a malate kinase,
- a second step of transforming 4-phospho-malate into malate-4-semialdehyde using a malate semialdehyde dehydrogenase
- a third step of transforming malate-4-semialdehyde into 2,4-DHB using a DHB dehydrogenase.
A malate kinase wherein it transforms malate into 4-phospho-malate.
A malate semialdehyde dehydrogenase wherein it transforms 4-phospho-malate into malate-4-semialdehyde.
A DHB dehydrogenase wherein it transforms malate-4-semialdehyde in 2,4-DHB.
A host microorganism expressing a malate kinase, and/or a malate semialdehyde dehydrogenase and/or a DHB dehydrogenase.
The process of production of 2,4 DHB comprising a first step of production of malate or other organic acid by a malate overproducing microorganism.
A method of producing 2,4-DHB comprising:
- transforming malate-4-semialdehyde by a methylbutyraldehyde reductase or a succinic semialdehyde reductase
4-phospho-malate.
Dependencies are inferred from the claim text.