Back to results
US 2013/0273616 A1Published application
Polypeptides and biosynthetic pathways for the production of monatin and its precursors
AssigneeCargill, Incorporated
InventorsPaula M. HICKS, Sara C. MCFARLAN
Life of this patent
Estimating the term…
Abstract
Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.
Claims7
A method for producing monatin or salt thereof comprising:
- (a) contacting a cell with tryptophan, wherein the cell expresses
- (i) a first polypeptide that facilitates the conversion of tryptophan to indole-3-pyruvate;
- (ii) a second polypeptide that facilitates the conversion of indole-3-pyruvate to 2-hydroxy2-(indol-3-ylmethyl)-4-keto glutaric acid, wherein the second polypeptide is an aldolase;
- and
- (iii) a third polypeptide that facilitates the conversion of 2-hydroxy2-(indol-3-ylmethyl)-4-keto glutaric acid to monatin or salt thereof;
- wherein at least one of the first and third polypeptides are selected from the group consisting of HEXAspC aminotransferase (SEQ ID NO:76) and YfdZ (SEQ ID NO:92);
- (b) culturing the cell under conditions whereby monatin or salt thereof is produced;
- and
- (c) harvesting monatin or salt thereof.
6 more elements
Dependencies are inferred from the claim text.