Method of Manufacturing Nanoparticle Chain

A method of manufacturing a nanoparticle chain, comprising the steps of:

• providing at least one single-stranded DNA nanotemplate, and an end of the at least one single-stranded DNA nanotemplate being fixed to a surface of a substrate, and the at least one single-stranded DNA nanotemplate having a secondary structure;
• adding a plurality of first single-stranded DNA probes, and an end of each of the first single-stranded DNA probes being combined with a nanoparticle and attached to a sequence corresponding to the at least one single-stranded DNA nanotemplate to form at least one nanoparticle chain having the secondary structure;
• adjusting a predetermined reaction temperature to change the secondary structure of the at least one nanoparticle chain;
• and
• adding a plurality of second single-stranded DNA probes attached to a sequence corresponding to the at least one single-stranded DNA nanotemplate;
• thereby, the predetermined reaction temperature is adjusted to control the secondary structure of the at least one single-stranded DNA nanotemplate in order to control an inter-nanoparticle distance of the nanoparticle chain.

A method of manufacturing a nanoparticle chain, comprising the steps of:

• providing at least one single-stranded DNA nanotemplate, and an end of the at least one single-stranded DNA nanotemplate being fixed to a surface of a substrate;
• and
• adding a plurality of first single-stranded DNA probes and a plurality of second single-stranded DNA probes, and an end of each of the first single-stranded DNA probes being combined to a nanoparticle, and the second single-stranded DNA probes not combined with the nanoparticle, the first single-stranded DNA probes and the second single-stranded DNA probes competitively attached to a sequence corresponding to the at least one single-stranded DNA nanotemplate to form at least one nanoparticle chain;
• thereby, the first single-stranded DNA probes and the second single-stranded DNA probes have the sequences, and a ratio of the first single-stranded DNA probes to the second single-stranded DNA probes is adjusted to control a quantity of the nanoparticles attached to the at least one single-stranded DNA nanotemplate, so as to control an inter-nanoparticle distance of the nanoparticle chain.