Strains of s.cerevisiae capable of growing in media with melibiose, stachyose and raffinose
OwnerManuel Becerra Fernández, María Esperanza Cerdán Villanueva +3inventors
InventorsManuel Becerra Fernández, María Esperanza Cerdán Villanueva, Rafael Fernández Leiro, María Isabel González Siso, Ángel Pereira Rodríguez
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Abstract
The invention relates to strains of S. cerevisiae capable of secreting α-galactosidase into culture medium, as well as to methods for obtaining α-galactosidase using said strains and methods for producing biomass and bioethanol by means of culturing said strains in media rich in galactose. In another aspect, the invention provides methods for expressing recombinant proteins using a composition rich in galactose as a culture medium for the microorganisms producing said protein.
Claims23
1-19. (canceled)
A DNA construct comprising:
- (a) a heterologous promoter regulated by means of glucose repression,
- (b) a sequence encoding a functional signal sequence in yeast, and
- (c) the region of the MEL1 gene encoding the mature form of α-galactosidase or a functionally equivalent variant thereof,
- wherein sequences (b) and (c) are under the same reading frame.
A DNA construct comprising:
- (a) the ADH2 promoter or a functionally equivalent variant thereof,
- (b) a sequence encoding a functional signal sequence in yeast, and
- (c) the region of a gene encoding a mature form of α-galactosidase or a functionally equivalent variant thereof,
- wherein sequences (b) and (c) are under the same reading frame.
Dependencies are inferred from the claim text.