Pentose and glucose fermenting yeast cell
AssigneeDsm IP Assets B.V.
InventorsHendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
Life of this patent
Estimating the term…
Abstract
The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.
Claims12
A yeast cell comprising at least one exogenous gene of at least one pentose metabolic pathway non-native to the yeast cell wherein said yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell.
A process for preparing a pentose fermenting yeast cell, comprising subjecting a yeast cell comprising at least one exogenous gene of pentose metabolic pathway to disruption of the gene hxk2 native in the yeast cell, and subjecting a resulting disruptant strain to evolutionary engineering to improve pentose consumption, until the yeast cell has growth rate of at least 0.05 h-1 on the pentose as sole carbon source and isolating the resulting pentose fermenting yeast cell.
The process according to claim 7, wherein the fermentation time is reduced relative to the corresponding fermentation of wild-type yeast.
The process according to claim 8, wherein the fermentation time is reduced at least 40% or more.
Dependencies are inferred from the claim text.