Ambient temperature stable kits for molecular diagnostics
AssigneeMolecular Detection, Inc.
InventorsBoaz ARIELI, Fanny SZAFER SHKEDY, Vered ROITMAN, Giora BAR-AKIVA, Aryeh GASSEL
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Abstract
A method for processing DNA polymerase and/or dNTPs for use in an amplification procedure, includes providing a solution mixture, the solution mixture including a DNA polymerase and/or dNTPs, a buffer solution and at least one stabilizing agent and hydration reducing the solution mixture. The solution mixture is hydration reduced at a temperature between 0° C. and about 100° C.
Claims21
A method for processing DNA polymerase and/or dNTPs for use in an amplification procedure, the method comprising:
- providing a solution mixture, the solution mixture including a DNA polymerase and/or dNTPs, a buffer solution and at least one stabilizing agent;
- and
- hydration reducing the solution mixture, wherein the provided solution mixture is hydration reduced at a temperature between 0° C. and about 100° C.
A kit for the amplification of a nucleic acid, said kit comprising a hydration reduced solution comprising a thermophilic DNA polymerase and dNTPs, a buffer solution, at least one stabilizing agent, magnesium chloride, a set of two oligonucleotide primers, said oligonucleotide primers differing in sequence from each other and an oligonucleotide probe that differs in sequence from said set of two oligonucleotide primers, with or without a nucleic acid template.
A kit for the amplification and detection of nucleic acids, said kit comprising:
- a. a solution comprising a thermophilic DNA polymerase and dNTPs, a buffer solution, at least one stabilizing agent, magnesium chloride, a set of two oligonucleotide primers, said oligonucleotide primers differing in sequence from each other,
- b. at least one additional set of oligonucleotide primers, said oligonucleotide primers differing in sequence from each other and being capable of amplifying a region of target DNA that is distinct from the region of target that may be amplified by the first set of oligonucleotide primers,
- wherein the reagents of the kit are hydration reduced by means of drying at elevated temperatures, lyophilization, vacuum hydration removal, spray drying, fluidized bed drying or drum drying, and wherein the kit reagents are capable of nucleic acid amplification after having been stored at ambient temperature for up to 90 days and subsequently rehydrated.
A kit for the amplification and detection of nucleic acids, the kit comprising:
- a. a first set of two oligonucleotide primers, one or both of which is fluorescent labeled and/or an additional third oligonucleotide that is a labeled probe, said oligonucleotide primers and probe differing in sequence from each other, and able to detect in a quantitative PCR reaction the presence of a unique nucleic acid sequence,
- b. a second set of oligonucleotide primers, one or both of which is fluorescent labeled to serve as a probe or in additional to a third oligonucleotide serving as a probe, said second set of oligonucleotide primers and probe differing in sequence from each other, and able to detect in a quantitative PCR reaction the presence of a distinct nucleic acid sequence that is different from the nucleic acid sequence being detected by the first set of primers and probe,
- c. a DNA polymerase enzyme;
- d. dNTPs (dATP, dCTP, dGTP and dTTP);
- e. a buffer solution containing one or more stabilizing agents;
- and
- f. magnesium chloride,
- g. with or without a DNA template to serve as an internal control,
- wherein at least some of the reagents of the kit, including the DNA polymerase enzyme and the dNTPs, are hydration reduced by means of drying at elevated temperatures, lyophilization, vacuum hydration removal, spray drying, fluidized bed drying or drum drying and wherein the kit reagents together in a single mixture are capable of nucleic acid amplification activity after having been stored at ambient temperatures for up to 90 days and subsequently rehydrated.
Dependencies are inferred from the claim text.