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US 2013/0260464 A1Published application
Crosslinked hyaluronan hydrogels for 3d cell culture
AssigneeUniversite De Rouen
InventorsJean-Pierre Vannier, Laurent David, Didier Lecerf, Virginie Dulong, Berenice Coquerel, Elise Demange
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Abstract
The present invention relates to a new method for treating or processing a cross-linked hyaluronan hydrogel prior to its use in applications involving three-dimensional cell culture. The invention also relates to cross-linked hyaluronan hydrogels prepared by such a method and to their use in 3D cell culture, stem cell/tissue engineering, drug discovery, toxicology testing, and broad cell biology applications.
Claims25
A method for obtaining a cross-linked hyaluronan hydrogel suitable for 3D cell culture, said method comprising steps of:
- (a) lyophilizing the cross-linked hyaluronan hydrogel to obtain a lyophilized hyaluronan hydrogel or providing a lyophilized hyaluronan hydrogel;
- and
- (b) sterilizing the lyophilized hyaluronan hydrogel
- first by heating the hydrogel, and
- then by immersing the hydrogel in alcohol and submitting the immersed hydrogel to physical compression.
2 more elements
Dependencies are inferred from the claim text.