Thermal cycler and control method of thermal cycler

A thermal cycler comprising:

• an attachment unit having an insertion opening for insertion of a reaction container including a channel filled with a reaction solution containing reverse transcriptase enzyme and a liquid having a lower specific gravity than that of the reaction solution and being immiscible with the reaction solution, the reaction solution moving close to opposed inner walls;
• a first heating unit that heats a first region of the channel when the reaction container is attached to the attachment unit;
• a second heating unit that heats a second region of the channel nearer the insertion opening than the first region when the reaction container is attached to the attachment unit;
• a drive mechanism that switches arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement;
• and
• a control unit that controls the first heating unit and the second heating unit,
• wherein the first arrangement is an arrangement in which the first region is located in a lowermost part of the channel in a direction in which gravity acts when the reaction container is attached to the attachment unit,
• the second arrangement is an arrangement in which the second region is located in the lowermost part of the channel in the direction in which the gravity acts when the reaction container is attached to the attachment unit, and
• the control unit performs first processing of controlling a temperature of the first heating unit to be a temperature at which the reverse transcriptase enzyme has activity and controlling a temperature of the second heating unit to be a temperature at which the reverse transcriptase enzyme is not deactivated.