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US 2013/0260409 A1Published application
Store-operated calcium cellular assay
AssigneeNational Center for Biological Sciences
InventorsGayatri Venkiteswaran, Gaiti Hasan
Life of this patent
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Abstract
The present technology provides a cell based assay for identifying compounds that modulate store-operated ionic calcium levels using itpr mutant cell lines, such as itpr-ku cells, which have abnormal ionic calcium levels.
Claims8
1-10. (canceled)
A kit for identifying a compound that modulates store-operated calcium entry (SOCE) level in a cell, comprising:
- at least one mutant mammalian or avian cell, wherein the mutant mammalian or avian cell comprises a residual SOCE level and expresses a single 1,4,5-inositol triphosphate receptor (InsP3R) gene (itpr) product, wherein the itpr gene is an itprka1091/ug3 mutant, and wherein the itprka1091/ug3 product contains G1891S and S224F point mutations;
- and
- at least one control cell.
Dependencies are inferred from the claim text.