Generation of virosome particles
AssigneeFranvax S.R.L.
InventorMaria Di Naro
Life of this patent
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Abstract
The invention relates to the generation of a new class of virosome particles, making use of virus antigens expressed in plant, particularly influenza antigens, and to vaccines, particularly influenza vaccines, containing these virosome particles.
Claims19
-11. (canceled)
A synthetically produced virosome particle comprising (i) an influenza hemagglutinin (HA) antigen produced recombinantly in tobacco plants and (ii) a lipid bilayer, wherein the lipid bilayer comprises at least one bisacyloxypropylcysteine conjugate anchored in the lipid bilayer, and wherein said influenza hemagglutinin (HA) antigen is biologically active.
A vaccine comprising a plurality of virosome particles according to claim 1 and optionally a pharmacologically acceptable substance diluent.
A method of producing virosome particles, comprising:
- a) providing an influenza hemagglutinin (HA) antigen that has been recombinantly produced in plants;
- b) providing a mixture of phospholipids, wherein the mixture of phospholipids comprises at least one bisacyloxypropylcysteine conjugate;
- c) mixing together the influenza hemagglutinin (HA) antigen and the mixture of phospholipids;
- and
- d) reconstituting the influenza hemagglutinin (HA) antigen with the mixture of phospholipids to form said virosome particles.
Dependencies are inferred from the claim text.