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US 2013/0259864 A1Published application
Anti-Cobra Toxin Antibody Fragments and Method of Producing a VHH Library
AssigneeUniversity of Guelph
InventorsJ. Christopher Hall, Gabrielle Richard, Michael D. McLean
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Abstract
A method of constructing a VHH library from an immunized camelid, using whole venom or an extract thereof. There is also provided VHH antibody fragments isolated from a library produced in hyperimmunized llama. These VHH antibody fragments were sequenced, and specifically bind to α-cobratoxin.
Claims17
An isolated VHH antibody fragment comprising a heavy chain complementarity determining region 1 (CDR1) comprising the amino acid sequence selected from SEQ ID NOs:6-8 and 31;
- a heavy chain complementarity determining region 2 (CDR2) comprising the amino acid sequence selected from SEQ ID NOs:10, 18, 30 and 33;
- and a heavy chain complementarity determining region 3 (CDR3) comprising the amino acid sequence selected from SEQ ID NOs:12-13, 29 or 32.
A method of obtaining a VHH library comprising:
- (1) immunizing a camelid with whole venom or an extract thereof;
- (2) isolating nucleic acid sequences encoding the variable heavy fragment from the immunized camelid;
- and
- (3) transforming a suitable host with the nucleic acid sequences to prepare a recombinant VHH library comprising VHH antibody fragments that can bind to one or more proteins in the venom.
1 more element
Dependencies are inferred from the claim text.