US 2013/0259809 A1 · USE OF POLYSACCHARIDES FROM RADIX ISATIDIS IN MANUFACTURE OF MEDICAMENTS AGAINST INFLUENZA VIRUS

Use of polysaccharides from radix isatidis in manufacture of medicaments against influenza virus

AssigneesState Key Laboratory of Respiratory Diseases, Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd.

InventorsZifeng YANG, Chuyuan LI, Yutao WANG, Suishan ZHAO, Nanshan ZHONG, Qing LIN, Zheng WANG, Sheng QIN, Wenda GUAN, Ziyao MO, Deqin WANG

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Abstract

A process of using Isatis root polysaccharide in the manufacture of medicaments for treating and/or preventing diseases induced by influenza virus and complications thereof, wherein the molecular weight of the Isatis root polysaccharide is 3000-7000 Da, and the influenza viruses include influenza virus A, influenza virus B, avian influenza virus, such as human influenza virus H1N1, H3N2, avian influenza virus H6N2, H7N3, H9N2 and INF B. The mechanism of the Isatis root polysaccharide against influenza virus is due to the ability to inhibit the attachment of influenza virus to the host cell. The present invention also discloses a pharmaceutical composition containing the above-mentioned Isatis root polysaccharides and a method for preparing the polysaccharides.

Claims12

Dependent claims

A process in preventing and/or treating diseases caused by influenza viruses and complications thereof, using Isatis root polysaccharide, wherein the molecular weight of the Isatis root polysaccharide is 3000-7000 Dalton.

A pharmaceutical composition for preventing and/or treating diseases caused by influenza viruses and complications thereof, comprising Isatis root polysaccharide with the molecular weight of 3000-7000 Dalton;

and a dosage form, wherein the influenza viruses are at least one member selected from the group consisting human influenza A, human influenza B, human influenza virus subtypes H1N1 and H3N2, and avian influenza virus subtypes H6N2, H7N3, and H9N2.

A method for preparing the Isatis root polysaccharide, comprising:

A. decocting the Radix Isatidis raw materials by adding suitable times of distilled water to produce a decocted fluid, concentrating the decocted fluid to produce a concentrated solution so that the baume degree of which is 18-20° Bé at 50° C.;
B. cooling the concentrated solution obtained in Step A to below 45° C., adding alcohol to produce an alcohol solution so that alcohol content is up to 60% or above 60% in the alcohol solution, allowing the alcohol solution to stand for more than 12 hours to precipitate;
C. passing the alcohol solution through a macroporous resin column, eluting with pure water, and collecting an eluent;
D. deproteinizing the eluent, and placing the deproteinized solution in a dialysis bag with a proper molecular weight to dialyse;
and
E. evaporating the solution inside and/or outside the dialysis bag with a rotary evaporator to concentrated solution, and then freeze-drying the resulted components with a vacuum freeze drier to obtain components of the Isatis root polysaccharide with proper molecular weight.

3 more elements

Dependencies are inferred from the claim text.