Serum amyloid p protein
AssigneeLaboratoire Francais Du Fractionnement Et Des Biotechnologies
InventorsCatherine Chenou, Jean-François Prost
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Abstract
A method for purifying serum amyloid P protein from fresh unfrozen plasma, includes recalcifying the fresh unfrozen plasma and separation by phenyl-type hydrophobic interaction chromatography. The serum amyloid P protein is also obtainable by the method and the use thereof for therapeutic treatment purposes. A method for determining amyloid deposits in a tissue or organ of a subject is also provided.
Claims22
A method for purifying serum amyloid P protein from a starting material, the method comprising the following steps:
- a) a recalcification step;
- and
- b) a separation step by hydrophobic interaction chromatography.
A method for determining the presence of amyloid deposits in a tissue or organ of a subject, the method comprising:
- i. systemic administering of an amyloid P protein by radiolabelling the protein with at least one of:
- iodine 125 (125I), iodine 123 (123I), iodine 131 (131I), iodine 124 (124I), indium 111 (111In) or technetium 99m. (99 mTc);
- ii. using a gamma camera, detecting a signal in the tissue or organ, the signal being emitted by the protein;
- and
- iii. obtaining at least one image of the tissue or organ, the image being obtained as output data from a gamma camera.
A method for determining the presence of amyloid deposits in a tissue or organ in a subject, the method comprising:
- i. systemic administering of an amyloid P protein by radiolabelling the protein with at least one of:
- iodine 125 (125I), iodine 123 (123I), iodine 131 (131I), iodine 124 (124I), indium 111 (111In) or technetium 99m. (99 mTc);
- ii. quantifying, in a biological sample of the subject, the signal emitted by the administered protein;
- and
- iii. determining a ratio between the quantity of protein quantified at step (ii) and the quantity of protein administered at step (i).
Dependencies are inferred from the claim text.