Electrode for biosensor for lps detection, a method for producing it and a method for regenerating it
AssigneeResearch & Business Foundation Sungkyunkwan University
InventorsYoung Kwan Lee, Mi Suk Cho, Li Min Chun
Life of this patent
Estimating the term…
Abstract
A method of forming a biosensor electrode for lipopolysaccharide (LPS) detection includes providing a metal electrode, forming a metal electrode/3-mercaptopropionic acid (MPA) by depositing MPA on the metal electrode, forming a metal electrode/MPA/n-hydroxy-succinimide (NHS) by ester-bonding NHS and the MPA on the metal electrode/MPA, forming a metal electrode/MPA/nitrilotriacetic acid (NTA) by amide-bonding NTA-C4—NH2 and the MPA of the metal electrode/MPA/NHS, and forming a metal electrode/MPA/NTA/Cu by coordinate-bonding Cu2+ and the NTA of the metal electrode/MPA/NTA.
Claims13
A method of forming a biosensor electrode for lipopolysaccharide (LPS) detection, comprising:
- providing a metal electrode;
- forming a metal electrode/3-mercaptopropionic acid (MPA) by depositing MPA on the metal electrode;
- forming a metal electrode/MPA/n-hydroxy-succinimide (NHS) by ester-bonding NHS and the MPA on the metal electrode/MPA;
- forming a metal electrode/MPA/nitrilotriacetic acid (NTA) by amide-bonding NTA-C4—NH2 and the MPA of the metal electrode/MPA/NHS;
- and
- forming a metal electrode/MPA/NTA/Cu by coordinate-bonding Cu2+ and the NTA of the metal electrode/MPA/NTA.
A biosensor electrode for lipopolysaccharide (LPS) detection, comprising:
- a metal electrode on which NTA-C4—NH2 coordinated with Cu2+ is coupled.
A method of forming a biosensor electrode for lipopolysaccharide (LPS) detection, comprising:
- providing a metal electrode;
- forming a metal electrode/3-mercaptopropionic acid (MPA) by immersing the metal electrode in a solution in which MPA is dissolved in an ethanol-water solvent;
- forming a metal electrode/MPA/n-hydroxy-succinimide (NHS) by immersing the metal electrode/MPA in a solution in which 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and NHS are dissolved in a 2-(N-morpholino)ethanesulfonic acid (MES) solvent;
- forming a metal electrode/MPA/nitrilotriacetic acid (NTA) by immersing the metal electrode/MPA/NHS in a solution in which NTA-C4—NH2 is dissolved in MES solvent;
- and
- forming a metal electrode/MPA/NTA/Cu by immersing the metal electrode/MPA/NTA in a NaAc/HAc solution containing CuCl2.
Dependencies are inferred from the claim text.