Method of Use of Substrate Having Properties of Keratinous Tissue

A method of modeling the deposition of a substance onto mammalian keratinous tissue, comprising the steps of:

• a) applying a substance to a surface of an artificial substrate to form a substance-coated surface, wherein the substrate surface demonstrates at least one physical property selected from the group consisting of a total surface energy of from about 15 mJ/m2 to about 50 mJ/m2, a polar component of the total surface energy of from about 0 mJ/m2 to about 15 mJ/m2, a zeta-potential at a pH of about 5.0 of from about −30 mV to about 30 mV, texture that mimics mammalian keratinous tissue, and combinations thereof;
• and
• b) assessing the retention of substance on the surface of the substrate.

A method of modeling adhesion of an adhesive article of manufacture onto keratinous tissue, comprising:

• a) placing the article of manufacture in contact with an artificial substrate to form a substrate-adhesive complex, wherein the substrate surface demonstrates at least one physical property selected from the group consisting of a total surface energy of from about 15 mJ/m2 to about 50 mJ/m2, a polar component of the total surface energy of from about 0 mJ/m2 to about 15 mJ/m2, a zeta-potential at a pH of about 5.0 of from about −30 mV to about 30 mV, texture that mimics mammalian keratinous tissue, and combinations thereof;
• and
• b) performing at least one analysis of the substrate-adhesive complex.