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US 2013/0230896 A1Published application
Covalently Immobilized Enzyme and Method To Make The Same
AssigneeFresenius Medical Care Holdings, Inc.
InventorStephen A. Merchant
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Abstract
A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.
Claims24
A method for preparing an immobilized enzyme, the method comprising:
- forming an aqueous mixture of at least one polymer and at least one enzyme;
- adding at least one crosslinker to the aqueous mixture to form a reaction mixture;
- and
- maintaining the reaction mixture to immobilize the enzyme in a formation of covalently bound macromolecules.
1 more element
24-51. (canceled)
Dependencies are inferred from the claim text.